Drosophila melanogaster (D. melanogaster), commonly known as the fruit fly, has been a pivotal organism in genetic, developmental, and aging research. Its short life cycle, well-mapped genome, and ease of handling make it an ideal model for lifespan studies. Lifespan is often considered the ultimate biomarker of aging because it integrates the cumulative effects of various aging processes at the molecular, cellular, and systemic levels. It serves as a direct and quantifiable measure of the aging process, making it a standard for evaluating the efficacy of interventions aimed at extending healthy life expectancy.
Fig. 1 D. melanogaster life cycle at 25°C. They live for 60-90 days depending on the rearing conditions (i.e., temperature and diet composition). (Brischigliaro M, et al., 2023)
CD BioSciences offers comprehensive Drosophila lifespan analysis services to support the growing field of aging research. Our specialized services are designed to provide clients with the insights needed to advance their studies.
We work with clients to design experiments that address their specific research questions. Our experts assist clients in selecting the appropriate Drosophila strains, determining environmental conditions, and establishing the number of replicates needed to achieve statistical significance. We help our clients prepare Drosophila media with suitable humidity containing glucose, agar, yeast, corn starch, propionic acid, and other ingredients.
Our trained technicians maintain Drosophila cultures under controlled conditions to ensure optimal growth and health. We conduct lifespan assays by monitoring flies daily. If a fly sticks to the wall of a food container or bottle, and won't come off when you slam the old bottle on top of the new one, it is considered dead. Our experts also pay attention to environmental factors such as temperature, humidity, and light cycles, all of which can influence lifespan.
Our team of scientists records the survival of individual flies throughout the experiment. Our data analysis services include survival curve plotting, median lifespan calculation, and statistical evaluation to identify significant differences between experimental groups.
With the collected data, we utilize advanced statistical methodologies to analyze the lifespan data. We employ Kaplan-Meier survival curves to visualize the survival of different experimental groups. Additionally, we conduct log-rank tests to compare survival distributions, providing clients with insights into the significance of observed differences.
At CD BioSciences, we not only analyze individual lifespan data, but also facilitate comparative analyses between various Drosophila strains. Our services enable clients to identify significant factors influencing longevity and to understand the underlying biological mechanisms.
CD BioSciences provides customized reporting services to ensure the clarity and utility of the data. Our reports include detailed statistical analyses and graphical representations of survival data.
CD BioSciences provides a comprehensive and tailored suite of Drosophila lifespan analysis services. Our expertise in experimental design, meticulous data collection, and sophisticated data analysis enables clients to make significant contributions to the field of aging research. If you are interested in our services, please feel free to contact us or make an online inquiry.
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All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
CD BioScience is a contract research organization focused on aging research, specializing in providing research on aging mechanisms, preclinical drug discovery and development, and the development of healthcare and skincare products.
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