Dog Telomerase Reverse Transcriptase (TERT) ELISA Kit

INQUIRY

CAT. NO.: TK021

General Info
Size	96 Tests / 5x96 Tests
Product Overview	This immunoassay Kit enables the quantitative determination of target antigen concentrations in vitro, using serum, plasma, tissue homogenates, cell culture supernates, or other biological fluids as specimens. The microtiter plate provided with this Kit comes pre-coated with an antibody that specifically recognizes the target antigen. Following this, standards or samples are introduced into the respective microtiter plate wells, along with a biotin-conjugated antibody specific to the target antigen. Subsequently, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and allowed to incubate. The next step involves the addition of a TMB substrate solution to each well. Only those wells containing the target antigen, biotin-conjugated antibody, and enzyme-conjugated avidin will display a color change. To conclude the enzymatic reaction, a sulfuric acid solution is added, and the color shift is quantitatively measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of the target antigen in the samples is then determined by comparing the optical density (O.D.) of the samples to the standard curve.
Storage Conditions	Store at 4°C
Notes	Occasionally, trace amounts of reagent may remain in the closure of the product vial during shipment and storage of the TERT ELISA kit. It is recommended that any liquid that may be present in the lid of the container be centrifuged to the bottom using a bench-top centrifuge prior to experimentation.
Reactivity	Dog
Specificity	Recombinant and natural Dog Telomerase reverse transcriptase.
Shipping Conditions	Dry Ice

Product Details
Assay Type	Sandwich
Samples	Serum, Plasma, Tissue Homogenates, Other Biological Fluids
Detection Range	78-5000 pg/mL
Detection Wavelength	450 nm
Assay Time	3-5 h

Target Info
Molecular Weight	124,825 Da
NCBI Accession	NP_001026800.1
NCBI GenBank Nucleotide	NM_001031630.1
NCBI GeneID	403412
NCBI Official Full Name	telomerase reverse transcriptase
NCBI Official Symbol	TERT
NCBI Protein Information	telomerase reverse transcriptase
UniProt Entry Name	TERT_CANLF
UniProt Gene Name	TERT
UniProt Protein Name	Telomerase reverse transcriptase
UniProt Synonym Protein Names	Telomerase catalytic subunit
UniProt Primary Accession	Q6A548
Protein Family	Telomerase reverse transcriptase
UniProt Comments	Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis ().

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