Customization Services for Senescent Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are mesoderm-derived progenitor cells that have fibroblast-like morphology, adhere to a tissue culture flask, express a specific set of surface CD markers, and differentiate into osteocytes, adipocytes, and chondrocytes. With aging, a portion of cells, including MSCs, become senescent, and these senescent cells accumulate and promote various age-related diseases.

Fig. 1 Effect of senescent microenvironment on MSCs. (Chen H, et al., 2022)

Culturing senescent MSCs provides an opportunity to understand the molecular and cellular changes that occur during the aging process. CD BioSciences offers customization services for culturing senescent MSCs. Our customization services for senescent MSC serve as a valuable tool for drug screening and therapeutic development in the field of aging-related diseases.

Modeling Methods for Senescent MSCs

Long culture passage induction of MSCs

We have developed reliable solutions that involve subjecting MSCs to extended culture periods, allowing them to undergo replicative senescence. During this process, MSCs experience a gradual loss of proliferative capacity and exhibit characteristic senescence-associated phenotypic changes. Through our expertise and culturing techniques, we optimize culture conditions, such as media composition, supplementation of growth factors, and oxygen tension, to promote the development of senescence in MSCs.

Microgravity induction of MSCs

Microgravity has been shown to affect cellular behavior and trigger senescence in various cell types. Utilizing bioreactor systems and simulating microgravity environments, CD BioSciences can culture MSCs under conditions that enable ground-based microgravity research. We also support customizing the culture conditions, including the duration of microgravity exposure and optimization of nutrient supply, to provide our clients with senescent MSCs.

Applications of Senescent MSCs in Aging-Related Diseases

The senescent phenotype of MSCs holds great potential for understanding and addressing aging-related diseases. CD BioSciences recognizes the importance of exploring the applications of senescent MSCs in various therapeutic areas.

• Senescent MSCs in age-related tissue regeneration. We offer customization services for culturing senescent MSCs to help clients investigate the regenerative potential of senescent cells in age-related tissue damage, such as osteoarthritis, neurodegenerative disorders, and cardiovascular diseases.
• Senescent MSCs in aging-related immunomodulation. Our customization services for culturing senescent MSCs assist clients in studying the impact of senescence on MSC-mediated immunomodulation in aging-related immune disorders, such as autoimmune diseases and chronic inflammation.
• Senescent MSCs in age-related cancer research. Our clients can employ our senescent MSCs in cancer research to investigate the interactions between senescent MSCs and cancer cells, tumor microenvironment remodeling, and the potential role of senescence-associated secretory phenotype (SASP) in age-related carcinogenesis.

Our Advantage

• Skilled scientific team. We have a team of experienced scientists and researchers with expertise in senescent MSCs, capable of developing innovative solutions and interpreting complex data.
• Advanced laboratory facilities. We have access to advanced laboratory equipment and facilities for senescent MSC culture, gene editing, and quality control assessments, ensuring the high quality of services provided.
• Quality control. Our implementation of robust quality management systems ensures the reliability, reproducibility, and compliance of customization services for senescent MSCs.

Leveraging the expertise of CD BioSciences and our culturing techniques, our clients can gain a deeper understanding of the phenotype of senescent MSCs and explore their potential in addressing aging-related diseases. Our commitment to scientific excellence and customer satisfaction makes us the ideal partner for customized services in culturing senescent MSCs. If you are interested in our services, please feel free to contact us or make an online inquiry.

References

1. Chen H, et al. Aging and Mesenchymal Stem Cells: Therapeutic Opportunities and Challenges in the Older Group. Gerontology, 2022, 68 (3): 339-352.
2. Veronesi F, et al. In Vitro Models of Cell Senescence: A Systematic Review on Musculoskeletal Tissues and Cells. Int J Mol Sci, 2023, 24 (21): 15617.