CAT. NO.: TK020
| General Info | |
|---|---|
| Size | 24 Tests / 48 Tests / 96 Tests / 5×96 Tests / 10×96 Tests |
| Application | Enzyme-linked immunosorbent assay for antigen detection. |
| Product Overview | This ELISA kit is designed for the quantitative assessment of chicken TERT concentrations in serum, plasma, and other biological fluids through in vitro analysis. It employs the Sandwich-ELISA method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TERT. To conduct the assay, standards or samples are introduced into the respective micro ELISA plate wells and mixed with the specific antibody. Then a biotinylated detection antibody specific for TERT and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Unbound components are then thoroughly washed away. Following this, a substrate solution is introduced to each well. Only those wells that contain TERT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzymatic reaction with the substrate is halted by adding a sulfuric acid solution, causing the color to change to yellow. The optical density (OD) is quantitatively measured using a spectrophotometer at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TERT. You can determine the TERT concentration in the samples by comparing their OD to the standard curve provided. |
| Storage Conditions | Store at 4°C |
| Notes | Occasionally, trace amounts of reagent may remain in the closure of the product vial during shipment and storage of the TERT ELISA kit. It is recommended that any liquid that may be present in the lid of the container be centrifuged to the bottom using a bench-top centrifuge prior to experimentation. |
| Reactivity | Chicken |
| Specificity | This kit recognizes natural and recombinant Chicken TERT. No significant cross-reactivity or interference between Chicken TERT and analogues was observed. |
| Shipping Conditions | Dry Ice |
| Product Details | |
|---|---|
| Assay Type | Sandwich |
| Samples | Serum, Plasma, Biological Fluids |
| Detection Range | 0.156-10 ng/mL |
| Sensitivity | Min: 0.094 ng/mL; Max: 10 ng/mL |
| Detection Wavelength | 450 nm |
| Target Info | |
|---|---|
| Molecular Weight | 88,965 Da |
| NCBI Accession | NP_937983.2 |
| NCBI GenBank Nucleotide | NM_198253.2 |
| NCBI GeneID | 7015 |
| NCBI Official Full Name | telomerase reverse transcriptase isoform 1 |
| NCBI Official Symbol | TERT |
| NCBI Official Synonym Full Names | telomerase reverse transcriptase |
| NCBI Official Synonym Symbols | TP2, TRT, CMM9, EST2, TCS1, hTRT, DKCA2, DKCB4, hEST2, PFBMFT1 |
| NCBI Protein Information | telomerase reverse transcriptase, telomerase catalytic subunit, telomerase-associated protein 2 |
| OMIM | 178500 |
| UniProt Entry Name | TERT_HUMAN |
| UniProt Gene Name | TERT |
| UniProt Protein Name | Telomerase reverse transcriptase |
| UniProt Synonym Gene Names | EST2, TCS1, TRT, TP2 |
| UniProt Synonym Protein Names | HEST2, Telomerase catalytic subunit, Telomerase-associated protein 2, TP2 |
| UniProt Primary Accession | O14746 |
| UniProt Secondary Accession | O14783, Q2XS35, Q8N6C3, Q8NG38, Q8NG46 |
| Protein Family | Telomerase reverse transcriptase |
| NCBI Summary | Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq, Jul 2008] |
| UniProt Comments | TERT: telomerase reverse transcriptase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The holoenzyme consists of TERT and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced isoforms have been identified, although the full-length sequence of some variants has not been determined.Protein type: EC 2.7.7.49; Transferase; Nucleolus; DNA-bindingChromosomal Location of Human Ortholog: 5p15.33Cellular Component: nucleoplasm; PML body; chromosome, telomeric region; nuclear telomere cap complex; telomerase holoenzyme complex; nucleolusMolecular Function: telomerase activity; protein binding; protein homodimerization activity; RNA-directed RNA polymerase activity; DNA binding; metal ion binding; telomeric DNA binding; RNA-directed DNA polymerase activity; telomeric template RNA reverse transcriptase activity; tRNA binding; nucleotidyltransferase activityBiological Process: mitochondrion organization and biogenesis; DNA strand elongation; RNA-dependent DNA replication; positive regulation of nitric-oxide synthase activity; telomere maintenance via telomerase; age-dependent telomere shortening; positive regulation of Wnt receptor signaling pathway; telomere maintenance; RNA interference, production of siRNADisease: Pulmonary Fibrosis, Idiopathic; Aplastic Anemia; Dyskeratosis Congenita, Autosomal Dominant, 1 |
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