CD19 (1D3) Rat Antibody (APC Conjugate)

INQUIRY

CAT. NO.: AMA-0799

Target Information
UniProt ID	P25918

Product Details
Size	100 µg
Reactivity	Mouse
Host	Rat
Source/Isotype	Rat IgG2a
Storage	Store at -20°C. Do not aliquot the antibody.
Purification	This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Usage
Application	F
Product Usage Information Dilution	Flow Cytometry (1:160)

Background
CD19 is a 95 kDa coreceptor which amplifies the signaling cascade in B cells On the B cell surface, CD19 associates with CD21, CD81, and Leu-13 to exert its function. The cytoplasmic tail of CD19 has nine conserved tyrosine residues playing critical roles in CD19 mediated function by coupling signaling molecules to the receptor After B cell receptor or CD19 ligation, Tyr531 and Tyr500 of CD19 are progressively phosphorylated. This phosphorylation enables the coupling of PI3 kinase and Src family tyrosine kinase to CD19 and activates the PI3K and Src signaling pathways Coligation of B cell receptor and CD19 also promotes Tyr409 phosphorylation in CD19. The phosphorylation at these sites enables its binding to Vav and mediates elevated intracellular calcium response, as well as the JNK pathwayThe 1D3 clone is a widely used as a phenotypic marker for CD19 expression on the surface of murine B cells and subsets of dendritic cells.

Background

CD19 is a 95 kDa coreceptor which amplifies the signaling cascade in B cells On the B cell surface, CD19 associates with CD21, CD81, and Leu-13 to exert its function. The cytoplasmic tail of CD19 has nine conserved tyrosine residues playing critical roles in CD19 mediated function by coupling signaling molecules to the receptor After B cell receptor or CD19 ligation, Tyr531 and Tyr500 of CD19 are progressively phosphorylated. This phosphorylation enables the coupling of PI3 kinase and Src family tyrosine kinase to CD19 and activates the PI3K and Src signaling pathways Coligation of B cell receptor and CD19 also promotes Tyr409 phosphorylation in CD19. The phosphorylation at these sites enables its binding to Vav and mediates elevated intracellular calcium response, as well as the JNK pathwayThe 1D3 clone is a widely used as a phenotypic marker for CD19 expression on the surface of murine B cells and subsets of dendritic cells.

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