Cathepsin B (mouse) ELISA Kit
Online Inquiry

Cathepsin B (mouse) ELISA Kit

INQUIRY

CAT. NO.: SAT-018

General Info
Size 100 tests
Product Overview Cathepsin B (mouse) ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody form rat specific for Cathepsin B coated on a 96-well plate. Standards (NSO, H18-F339) and test samples are added to the wells and Cathepsin B present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for Cathepsin B is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, Avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse Cathepsin B captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins.
Detection Range: 156 pg/mL – 10,000 pg/mL. Sensitivity: < 5 pg/mL.
Notes This ELISA kit is used for quantitative protein detection, establishing normal range.

Our customers have direct access to our experts and give timely feedback to any online inquiries. If you are interested in our products, please contact us.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.