Canine Telomerase Reverse Transcriptase (TERT) ELISA Kit

INQUIRY

CAT. NO.: TK014

General Info
Size	48-Strip-Wells / 96-Strip-Wells / 5×96-Strip-Wells / 10×96-Strip-Wells
Product Overview	This TERT ELISA Kit is designed for the quantitative determination of Canine TERT and employs a solid-phase ELISA format. The TERT ELISA Kit utilizes a competitive enzyme immunoassay approach, incorporating a polyclonal anti-TERT antibody and a TERT-HRP conjugate. During the assay, the sample and buffer are incubated together with the TERT-HRP conjugate on a pre-coated plate for one hour. Subsequently, the wells are decanted and washed five times. The next step involves incubation with an HRP enzyme substrate. The resulting enzyme-substrate reaction generates a blue-colored complex. To halt the reaction, a stop solution is added, turning the solution yellow. The intensity of the color is measured spectrophotometrically at 450nm using a microplate reader. The intensity of the color is inversely proportional to the concentration of TERT, as TERT from the samples competes with TERT-HRP conjugate for binding sites on the anti-TERT antibody. Since the binding sites are limited, as more sites are occupied by TERT from the sample, fewer sites remain available to bind TERT-HRP conjugate. A standard curve is constructed to establish the relationship between color intensity (O.D.) and the concentration of standards. The TERT concentration in each sample is then determined by interpolation from this standard curve.
Storage Conditions	Store all reagents at 2-8°C
Notes	Occasionally, trace amounts of reagent may remain in the closure of the product vial during shipment and storage of the TERT ELISA kit. It is recommended that any liquid that may be present in the lid of the container be centrifuged to the bottom using a bench-top centrifuge prior to experimentation.
Reactivity	Canine
Shipping Conditions	Dry Ice

Product Details
Assay Type	Competitive
Samples	Serum, Plasma, Cell Culture Supernatants, Body Fluid, Tissue Homogenate
Sample Preparation	We recommend preliminary experimentation using undiluted samples or dilutions at ratios of 1:2 or 1:4. Please refrain from exceeding a 1:10 dilution, as it surpasses the prescribed dilution threshold for this kit.
Sensitivity	1.0 pg/mL
Detection Wavelength	450 nm

Target Info
Molecular Weight	88,965 Da
NCBI Accession	O14746.1
NCBI GeneID	7015
NCBI Official Full Name	Telomerase reverse transcriptase
NCBI Official Symbol	TERT
NCBI Official Synonym Full Names	telomerase reverse transcriptase
NCBI Official Synonym Symbols	TP2, TRT, CMM9, EST2, TCS1, hTRT, DKCA2, DKCB4, hEST2, PFBMFT1
NCBI Protein Information	telomerase reverse transcriptase, telomerase catalytic subunit, telomerase-associated protein 2
OMIM	615134
UniProt Entry Name	TERT_HUMAN
UniProt Gene Name	TERT
UniProt Protein Name	Telomerase reverse transcriptase
UniProt Synonym Gene Names	EST2, TCS1, TRT, TP2
UniProt Synonym Protein Names	HEST2, Telomerase catalytic subunit, Telomerase-associated protein 2, TP2
UniProt Primary Accession	O14746
UniProt Secondary Accession	O14783, Q2XS35, Q8N6C3, Q8NG38, Q8NG46
Protein Family	Telomerase reverse transcriptase
NCBI Summary	Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq, Jul 2008]
UniProt Comments	TERT: telomerase reverse transcriptase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The holoenzyme consists of TERT and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced isoforms have been identified, although the full-length sequence of some variants has not been determined.Protein type: Nucleolus; EC 2.7.7.49; Transferase; DNA-bindingChromosomal Location of Human Ortholog: 5p15.33Cellular Component: nucleoplasm; PML body; chromosome, telomeric region; nuclear telomere cap complex; telomerase holoenzyme complex; nucleolusMolecular Function: telomerase activity; protein binding; RNA-directed RNA polymerase activity; protein homodimerization activity; DNA binding; metal ion binding; telomeric DNA binding; RNA-directed DNA polymerase activity; telomeric template RNA reverse transcriptase activity; nucleotidyltransferase activity; tRNA bindingBiological Process: mitochondrion organization and biogenesis; DNA strand elongation; RNA-dependent DNA replication; positive regulation of nitric-oxide synthase activity; telomere maintenance via telomerase; age-dependent telomere shortening; RNA interference, production of siRNA; telomere maintenance; positive regulation of Wnt receptor signaling pathwayDisease: Pulmonary Fibrosis, Idiopathic; Aplastic Anemia; Dyskeratosis Congenita, Autosomal Dominant, 1

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