CAT. NO.: TK014
| General Info | |
|---|---|
| Size | 48-Strip-Wells / 96-Strip-Wells / 5×96-Strip-Wells / 10×96-Strip-Wells |
| Product Overview | This TERT ELISA Kit is designed for the quantitative determination of Canine TERT and employs a solid-phase ELISA format. The TERT ELISA Kit utilizes a competitive enzyme immunoassay approach, incorporating a polyclonal anti-TERT antibody and a TERT-HRP conjugate. During the assay, the sample and buffer are incubated together with the TERT-HRP conjugate on a pre-coated plate for one hour. Subsequently, the wells are decanted and washed five times. The next step involves incubation with an HRP enzyme substrate. The resulting enzyme-substrate reaction generates a blue-colored complex. To halt the reaction, a stop solution is added, turning the solution yellow. The intensity of the color is measured spectrophotometrically at 450nm using a microplate reader. The intensity of the color is inversely proportional to the concentration of TERT, as TERT from the samples competes with TERT-HRP conjugate for binding sites on the anti-TERT antibody. Since the binding sites are limited, as more sites are occupied by TERT from the sample, fewer sites remain available to bind TERT-HRP conjugate. A standard curve is constructed to establish the relationship between color intensity (O.D.) and the concentration of standards. The TERT concentration in each sample is then determined by interpolation from this standard curve. |
| Storage Conditions | Store all reagents at 2-8°C |
| Notes | Occasionally, trace amounts of reagent may remain in the closure of the product vial during shipment and storage of the TERT ELISA kit. It is recommended that any liquid that may be present in the lid of the container be centrifuged to the bottom using a bench-top centrifuge prior to experimentation. |
| Reactivity | Canine |
| Shipping Conditions | Dry Ice |
| Product Details | |
|---|---|
| Assay Type | Competitive |
| Samples | Serum, Plasma, Cell Culture Supernatants, Body Fluid, Tissue Homogenate |
| Sample Preparation | We recommend preliminary experimentation using undiluted samples or dilutions at ratios of 1:2 or 1:4. Please refrain from exceeding a 1:10 dilution, as it surpasses the prescribed dilution threshold for this kit. |
| Sensitivity | 1.0 pg/mL |
| Detection Wavelength | 450 nm |
| Target Info | |
|---|---|
| Molecular Weight | 88,965 Da |
| NCBI Accession | O14746.1 |
| NCBI GeneID | 7015 |
| NCBI Official Full Name | Telomerase reverse transcriptase |
| NCBI Official Symbol | TERT |
| NCBI Official Synonym Full Names | telomerase reverse transcriptase |
| NCBI Official Synonym Symbols | TP2, TRT, CMM9, EST2, TCS1, hTRT, DKCA2, DKCB4, hEST2, PFBMFT1 |
| NCBI Protein Information | telomerase reverse transcriptase, telomerase catalytic subunit, telomerase-associated protein 2 |
| OMIM | 615134 |
| UniProt Entry Name | TERT_HUMAN |
| UniProt Gene Name | TERT |
| UniProt Protein Name | Telomerase reverse transcriptase |
| UniProt Synonym Gene Names | EST2, TCS1, TRT, TP2 |
| UniProt Synonym Protein Names | HEST2, Telomerase catalytic subunit, Telomerase-associated protein 2, TP2 |
| UniProt Primary Accession | O14746 |
| UniProt Secondary Accession | O14783, Q2XS35, Q8N6C3, Q8NG38, Q8NG46 |
| Protein Family | Telomerase reverse transcriptase |
| NCBI Summary | Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq, Jul 2008] |
| UniProt Comments | TERT: telomerase reverse transcriptase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The holoenzyme consists of TERT and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced isoforms have been identified, although the full-length sequence of some variants has not been determined.Protein type: Nucleolus; EC 2.7.7.49; Transferase; DNA-bindingChromosomal Location of Human Ortholog: 5p15.33Cellular Component: nucleoplasm; PML body; chromosome, telomeric region; nuclear telomere cap complex; telomerase holoenzyme complex; nucleolusMolecular Function: telomerase activity; protein binding; RNA-directed RNA polymerase activity; protein homodimerization activity; DNA binding; metal ion binding; telomeric DNA binding; RNA-directed DNA polymerase activity; telomeric template RNA reverse transcriptase activity; nucleotidyltransferase activity; tRNA bindingBiological Process: mitochondrion organization and biogenesis; DNA strand elongation; RNA-dependent DNA replication; positive regulation of nitric-oxide synthase activity; telomere maintenance via telomerase; age-dependent telomere shortening; RNA interference, production of siRNA; telomere maintenance; positive regulation of Wnt receptor signaling pathwayDisease: Pulmonary Fibrosis, Idiopathic; Aplastic Anemia; Dyskeratosis Congenita, Autosomal Dominant, 1 |
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