CAT. NO.: TK070
| General Info | |
|---|---|
| Size | 48-Strip-Wells / 96-Strip-Wells / 5×96-Strip-Wells / 10×96-Strip-Wells |
| Product Overview | This product constitutes a ready-to-use microwell and strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the assessment of the Protection of Telomeres 1 Homolog (POT1) ELISA Kit target analytes within biological samples. The concentration gradients of the Kit standards or positive controls render a theoretical Kit detection range in biological research samples containing POT1. The ELISA analytical biochemical technique of the Kit is based on POT1 antibody-POT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect POT1 antigen targets in samples. It's important to note that this ELISA Kit is specifically tailored for detecting native, not recombinant POT1. |
| Storage Conditions | Store all reagents at 2-8°C |
| Notes | Occasionally, trace amounts of reagent may remain in the closure of the product vial during shipment and storage of the POT1 ELISA kit. It is recommended that any liquid that may be present in the lid of the container be centrifuged to the bottom using a bench-top centrifuge prior to experimentation. |
| Reactivity | Bovine |
| Shipping Conditions | Dry Ice |
| Product Details | |
|---|---|
| Samples | Undiluted Body Fluids, Tissue Homogenates, Secretions |
| Target Info | |
|---|---|
| Molecular Weight | 71,442 Da |
| NCBI Accession | NP_056265.2 |
| NCBI GenBank Nucleotide | NM_015450.2 |
| NCBI GeneID | 25913 |
| NCBI Official Full Name | protection of telomeres protein 1 isoform 1 |
| NCBI Official Symbol | POT1 |
| NCBI Official Synonym Full Names | protection of telomeres 1 |
| NCBI Official Synonym Symbols | CMM10, HPOT1 |
| NCBI Protein Information | protection of telomeres protein 1, protection of telomeres 1 homolog, POT1-like telomere end-binding protein |
| OMIM | 606478 |
| UniProt Entry Name | POTE1_HUMAN |
| UniProt Gene Name | POT1 |
| UniProt Protein Name | Protection of telomeres protein 1 |
| UniProt Synonym Gene Names | hPot1 |
| UniProt Synonym Protein Names | POT1-like telomere end-binding protein |
| UniProt Primary Accession | Q9NUX5 |
| UniProt Secondary Accession | O95018, Q5MJ36, Q9H662, Q9NW19, Q9UG95 |
| Protein Family | Potassium transporter |
| NCBI Summary | This gene is a member of the telombin family and encodes a nuclear protein involved in telomere maintenance. Specifically, this protein functions as a member of a multi-protein complex that binds to the TTAGGG repeats of telomeres, regulating telomere length and protecting chromosome ends from illegitimate recombination, catastrophic chromosome instability, and abnormal chromosome segregation. Increased transcriptional expression of this gene is associated with stomach carcinogenesis and its progression. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008] |
| UniProt Comments | POT1: Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA- binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'- TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomere elongation by increasing telomerase processivity. Belongs to the telombin family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 7q31.33Cellular Component: nucleoplasm; chromosome, telomeric region; nuclear telomere cap complex; cytoplasm; nucleusMolecular protein binding; single-stranded telomeric DNA binding; DEAD/H-box RNA helicase bindingBiological Process: positive regulation of helicase activity; telomere assembly; negative regulation of telomerase activity; telomere capping; telomere maintenance via telomerase; positive regulation of telomerase activity; negative regulation of telomere maintenance via telomerase; telomere maintenance; DNA duplex unwinding; positive regulation of telomere maintenance via telomeraseDisease: Melanoma, Cutaneous Malignant, Susceptibility To, 10 |
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