CAT. NO.: AC-02007
| General Info | |
|---|---|
| Size | 50 tests/200 tests |
| Product Overview | The kit is designed for the extraction of exceptionally pure total DNA from a variety of sources, including fresh or frozen animal tissues, cells, blood, bacteria, and other samples. It allows for the purification of DNA fragments with a maximum molecular weight of 50 kb, all without the need for hazardous solvents such as phenol or chloroform, as well as ethanol precipitation. The optimized buffer system efficiently and specifically binds DNA from the lysate to a silica-based centrifugal adsorption column. Through a two-step washing process, PCR inhibitors and other substances that might interfere with enzymatic reactions can be effectively removed. Ultimately, high-purity DNA can be eluted using a low-salt buffer or water, making it ready for direct use in various downstream applications, including enzyme digestion, PCR, Real-Time PCR, library construction, Southern Blot, molecular markers, and other experiments. |
| Storage Conditions | Room temperature. |
| Notes | To maintain the quality of the samples and DNA extraction, it is essential to avoid repeated freezing and thawing, as this can lead to smaller DNA fragments and reduced yields. If you encounter a precipitate in Solution GA1, Solution GS, or Solution GA2, you can re-dissolve it by placing the solution in a 56°C water bath, shaking it, and then using it as needed. Additionally, please note that all centrifugation steps should be carried out using a benchtop centrifuge at room temperature. |
| Product Details | |
|---|---|
| Samples | Whole Blood, Cell Culture, Tissues, Bacteria, and Other Samples |
| Components | Proteinase K Solution GA1 Solution GS Solution GA2 Wash Buffer Solution RP Solution GE Adsorption column G column User manual |
| Highlights | 1. Rapid purification yields high-quality, ready-to-use DNA. 2. Good repeatability and high output. 3. Effectively remove pollutants and inhibitors, which is convenient for downstream applications. |
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