Assay Kits

Mitochondrial DNA (mtDNA) is circular, multicopy genome DNA located in mitochondrion, a cellular organelle that plays a key role in energy production of the cell. The capacity for energy production in a cell depends on both mtDNA integrity and copy number. Substantial evidence suggests that alterations in mtDNA copy number have been correlated with aging and various age-related disorders, such as cancer, diabetes and neurodegenerative diseases.

Mitochondrial DNA (mtDNA) is circular, multicopy genome DNA located in mitochondrion, a cellular organelle that plays a key role in energy production of the cell. The capacity for energy production in a cell depends on both mtDNA integrity and copy number. Substantial evidence suggests that alterations in mtDNA copy number have been correlated with aging and various age-related disorders, such as cancer, diabetes and neurodegenerative diseases.

Telomeres, consisting of repetitive nucleotide sequences, reside at the ends of chromosomes and serve as protective caps, shielding chromosomes from degradation and genetic information loss. In the natural course of cell division, normal diploid cells undergo telomere shortening with each cycle, leading to a gradual reduction in telomere length. This decline in telomere length has been associated with the potential to predict an organism's lifespan. Accurate and consistent quantification of telomere length is crucial in various facets of cell biology, including the study of chromosomal stability, DNA repair mechanisms, senescence (cellular aging), apoptosis (programmed cell death), cell dysfunctions, and the development of cancer. Mitochondrial DNA (mtDNA), a circular and multicopy genome, is located within the mitochondria, an essential cellular organelle responsible for energy production. The cell's capacity for energy production relies on both the integrity and copy number of mtDNA. There is evidence indicating a positive correlation between telomere length and mitochondrial dysfunction in the context of cellular aging and various age-related disorders, such as cancer, diabetes, and neurodegenerative diseases. A potential mechanistic link between these phenomena has been proposed, involving the PPAR signaling pathway. However, the precise details of this mechanism are still under investigation and require further elucidation.

Telomeres are repetitive nucleotide sequences located at the ends of chromosomes, and their primary role is to safeguard chromosomes from degradation and the loss of genetic information during cell division. In normal diploid cells, telomeres progressively shorten with each cell cycle. As a result, telomere length gradually decreases over time, and it has been associated with the potential to predict an organism's lifespan. The shortening of telomeres has detrimental effects on various aspects of health and is linked to numerous health conditions, including aging and cancer. Accurate and consistent quantification of telomere length is crucial in several areas of cell biology, including the study of chromosomal stability, DNA repair mechanisms, senescence (cellular aging), apoptosis (programmed cell death), cell dysfunctions, and the development of cancer. Understanding and quantifying telomere length provides valuable insights into these biological processes, contributing to our knowledge of how cells function and age, as well as their involvement in the onset and progression of diseases such as cancer.

Telomeres are repetitive nucleotide sequences located at the ends of chromosomes, and their primary role is to safeguard chromosomes from degradation and the loss of genetic information during cell division. In normal diploid cells, telomeres progressively shorten with each cell cycle. As a result, telomere length gradually decreases over time, and it has been associated with the potential to predict an organism's lifespan. The shortening of telomeres has detrimental effects on various aspects of health and is linked to numerous health conditions, including aging and cancer. Accurate and consistent quantification of telomere length is crucial in several areas of cell biology, including the study of chromosomal stability, DNA repair mechanisms, senescence (cellular aging), apoptosis (programmed cell death), cell dysfunctions, and the development of cancer. Understanding and quantifying telomere length provides valuable insights into these biological processes, contributing to our knowledge of how cells function and age, as well as their involvement in the onset and progression of diseases such as cancer.

Telomeres are repetitive nucleotide sequences located at the ends of chromosomes, and their primary role is to safeguard chromosomes from degradation and the loss of genetic information during cell division. In normal diploid cells, telomeres progressively shorten with each cell cycle. As a result, telomere length gradually decreases over time, and it has been associated with the potential to predict an organism's lifespan. The shortening of telomeres has detrimental effects on various aspects of health and is linked to numerous health conditions, including aging and cancer. Accurate and consistent quantification of telomere length is crucial in several areas of cell biology, including the study of chromosomal stability, DNA repair mechanisms, senescence (cellular aging), apoptosis (programmed cell death), cell dysfunctions, and the development of cancer. Understanding and quantifying telomere length provides valuable insights into these biological processes, contributing to our knowledge of how cells function and age, as well as their involvement in the onset and progression of diseases such as cancer.

Mitochondrial DNA (mtDNA) is circular, multicopy genome DNA located in mitochondrion, a cellular organelle that plays a key role in energy production of the cell. The capacity for energy production in a cell depends on both mtDNA integrity and copy number. Substantial evidence suggests that alterations in mtDNA copy number have been correlated with aging and various age-related disorders, such as cancer, diabetes and neurodegenerative diseases.

Mitochondrial DNA (mtDNA) is circular, multicopy genome DNA located in mitochondrion, a cellular organelle that plays a key role in energy production of the cell. The capacity for energy production in a cell depends on both mtDNA integrity and copy number. Substantial evidence suggests that alterations in mtDNA copy number have been correlated with aging and various age-related disorders, such as cancer, diabetes and neurodegenerative diseases.

Telomeres are repetitive nucleotide elements at the ends of chromosomes that protect chromosomes from degradation and genetic information loss. Normal diploid cells lose telomeres with each cell cycle. Telomere length, therefore, decreases over time and may predict lifespan. Accurate and consistent quantification of telomere length is important in many aspects of cell biology such as chromosomal instability, DNA repair, senescence, apoptosis, cell dysfunctions and oncogenesis. Mitochondrial DNA (mtDNA) is circular, multicopy genome DNA located in mitochondrion, a cellular organelle that plays a key role in the energy production. The capacity for energy production in a cell depends on both mtDNA integrity and copy number. Evidence suggests that telomere length and mitochondrial dysfunction are positively correlated in cellular aging and other age-related disorders such as cancer, diabetes and neurodegenerative diseases. A possible mechanistic link between them has been proposed through the PPAR signaling pathway. However, the specifics of the mechanism still have to be elucidated.

CD BioSciences' Telomere Length Test Kit (TK001) utilizes two-color fluorescence qPCR (SYRBGreen+VIC) to accurately measure relative telomere length in diverse human samples.

The quantitative detection of telomerase activity provides valuable information in many aspects such as cancer prognosis, treatment response, cellular aging, and potential therapeutic targets. The Telomerase Activity Test Kit enables the quantitative comparison of telomerase activity between cell populations. The kit utilizes mild lysis buffer to treat cells, allowing the native telomerase to be released. The telomere primer set is used to recognize and amplify newly synthesized telomere sequences in the assay, followed by the qPCR protocol to quantify telomerase activity in samples.

Accurate and consistent quantification of telomere length is essential in many aspects of cell biology, such as chromosomal instability, DNA repair, senescence, apoptosis, cell dysfunctions, and oncogenesis. This product utilizes relative quantitative PCR to determine the average telomere length of the given sample. The kit uses a single copy gene located on human chromosome 11 as the reference for data normalization. The relative telomere length is determined by calculating the copy number ratio (T/S) of telomere repeat sequences (Tel) to the single-copy reference (SCR).

The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of TE in the sample, this TE ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus TE concentration. The concentration of TE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

For the quantitative detection of Bovine Telomerase (TE) concentration in serum, plasma and other biological fluids. This assay employs a two-site sandwich ELISA to quantitate TE in Bovine serum, plasma. An antibody specific for TE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TE present is bound by the immobilized antibody. After removing any unbound substances, a biotinconjugated antibody specific for TE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TE bound in the initial step. The color development is stopped and the intensity of the color is measured.

This product constitutes a ready-to-use microwell and strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the assessment of the WD repeat containing, antisense to TP53 (WRAP53) ELISA Kit target analytes within biological samples. The concentration gradients of the Kit standards or positive controls render a theoretical Kit detection range in biological research samples containing WRAP53. The ELISA analytical biochemical technique of the Kit is based on WRAP53 antibody-WRAP53 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WRAP53 antigen targets in samples. It's important to note that this ELISA Kit is specifically tailored for detecting native, not recombinant WRAP53.

This product constitutes a ready-to-use microwell and strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the assessment of the Telomerase Protein Component 1 (TEP1) ELISA Kit target analytes within biological samples. The concentration gradients of the Kit standards or positive controls render a theoretical Kit detection range in biological research samples containing TEP1. The ELISA analytical biochemical technique of the Kit is based on TEP1 antibody-TEP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TEP1 antigen targets in samples. It's important to note that this ELISA Kit is specifically tailored for detecting native, not recombinant TEP1.

This product constitutes a ready-to-use microwell and strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the assessment of the Telomerase reverse transcriptase (TERT) ELISA Kit target analytes within biological samples. The concentration gradients of the Kit standards or positive controls render a theoretical Kit detection range in biological research samples containing TERT. The ELISA analytical biochemical technique of the Kit is based on TERT antibody-TERT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TERT antigen targets in samples. It's important to note that this ELISA Kit is specifically tailored for detecting native, not recombinant TERT.

This product constitutes a ready-to-use microwell and strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the assessment of the Telomerase Protein Component 1 (TEP1) ELISA Kit target analytes within biological samples. The concentration gradients of the Kit standards or positive controls render a theoretical Kit detection range in biological research samples containing TEP1. The ELISA analytical biochemical technique of the Kit is based on TEP1 antibody-TEP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TEP1 antigen targets in samples. It's important to note that this ELISA Kit is specifically tailored for detecting native, not recombinant TEP1.

This product constitutes a ready-to-use microwell and strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the assessment of the Telomerase Reverse Transcriptase (TERT) ELISA Kit target analytes within biological samples. The concentration gradients of the Kit standards or positive controls render a theoretical Kit detection range in biological research samples containing TERT. The ELISA analytical biochemical technique of the Kit is based on TERT antibody-TERT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TERT antigen targets in samples. It's important to note that this ELISA Kit is specifically tailored for detecting native, not recombinant TERT.

This product constitutes a ready-to-use microwell and strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the assessment of the Telomerase, ELISA Kit target analytes within biological samples. The concentration gradients of the Kit standards or positive controls render a theoretical Kit detection range in biological research samples containing TE. The ELISA analytical biochemical technique of the Kit is based on TE antibody-TE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TE antigen targets in samples. It's important to note that this ELISA Kit is specifically tailored for detecting native, not recombinant TE.