Assay Kits

Histone H4 Modification Multiplex Assay Kit (Colorimetric) is a complete set of optimized reagents to detect and quantify nearly all histone H4 modifications (10 different types) simultaneously in a simple, ELISA-like format with use of a standard microplate reader. Histone H4 Modification Multiplex Assay Kit is designed for measuring multiple histone H4 modifications simultaneously. In an assay with this kit, each histone H4 modified at specific sites will be captured by an antibody that is coated on the strip wells and specifically targets the appropriate histone H4 modification pattern. The captured histone modified at specific sites will be detected with a detection antibody, followed by a color development reagent. The ratio of modified histone is proportional to the intensity of absorbance measured by a microplate reader at a wavelength of 450 nm.

The TET Hydroxylase Activity Quantification Kit (Fluorometric) is suitable for measuring activity/inhibition of total 5mC hydroxylase TET enzyme using nuclear extracts or purified TET isoforms (TET 1-3) from a broad range of species such as mammalians, plants, fungi, and bacteria, in a variety of forms including, but not limited to cultured cells, fresh and frozen tissues.

DNMT1 (DNA (cytosine-5)-methyltransferase 1) belongs to the DNMT family, responsible for methylation of CpG islands in the genome. DNMT1 preferably methylates cytosines located in hemimethylated DNA and it associates with DNA replication sites in S-phase. DNMT1 Assay Kit allows the user to quickly and consistently measure the amount of DNMT1. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT1 assay experiment without the need for electrophoresis and transfer process. DNMT1 Assay Kit is suitable for measuring DNMT amounts quantitatively from fresh tissue and cultured cells of human and mouse.

DNMT3A (DNA (cytosine-5)-methyltransferase 3A) belongs to the DNMT family, and it is required for genome wide de novo methylation and it is essential for the establishment of DNA methylation patterns during development. DNMT3A Assay Kit allows the user to quickly and consistently measure the amount of DNMT3A. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT3A assay experiment. DNMT3A Assay Kit is suitable for measuring DNMT amounts quantitatively from fresh tissue and cultured cells of human and mouse.

DNMT3B (DNA (cytosine-5)-methyltransferase 3B) belongs to the DNMT family, it is required for genome de novo methylation and it is essential for establishment of DNA methylation patterns during development. DNMT3B Assay Kit allows the user to quickly and consistently measure the amount of DNMT3B. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT3B assay experiment. DNMT3B Assay Kit is suitable for quantitative measurement of DNMT3B amounts from fresh tissue and cultured cells of human and mouse.

m6A Demethylase Assay Kitis a complete set of optimized buffers and reagents to colorimetrically measure the activity/inhibition of total m6A demethylases using nuclear extracts or purified m6A demethylases like FTO and ALKBH5. m6A demethylase activity may be measured in a broad range of species such as mammalian, plant, fungal, and bacterial, in a variety of forms including, but not limited to, cultured cells and, fresh and frozen tissues. A unique m6A substrate is stably coated on the strip wells. Active m6A demethylases bind to and demethylate m 6A contained in the substrate. The un-demethylated m6A in the substrate can be recognized with a high affinity m6A antibody and the immuno-signal is enhanced with enhancer solution. The ratio or amount of undemethylated m6A, which is inversely proportional to enzyme activity, can then be colorimetrically quantified through an ELISA-like reaction.

Fast DNA modification Kit allows DNA denaturation and bisulfite conversion to be processed at the same time so the complete procedure can be performed in only 30 minutes. Furthermore, it prevents more than 90% of DNA loss, completely converting unmethylated cytosine into uracil. DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It is well demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases. Most methods for DNA methylation detection require a prior bisulfite-based DNA modification. Bisulfite-based DNA modification is used to discrimate between cytosine and methylated cytosine, in which DNA is treated with bisulfite salt to convert cytosine residues to uracil in a single-stranded DNA, while methylated cytosine remains the same. Not sure if this is the right kit for you? Check out our bisulfite modification guide for more information.

TET Hydroxylase Activity Quantification Kit (Colorimetric) is suitable for measuring the activity/inhibition of total 5mC hydroxylase TET enzyme using nuclear extracts or purified TET isoforms (TET 1-3) from a broad range of species such as mammalian, plant, fungal, and bacterial, in a variety of forms including, but not limited to cultured cells, fresh and frozen tissues.

DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It is well demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases. Most methods for DNA methylation detection require a prior bisulfite-based DNA modification. Bisulfite-based DNA modification is used to discrimate between cytosine and methylated cytosine, in which DNA is treated with bisulfite salt to convert cytosine residues to uracil in a single-stranded DNA, while methylated cytosine remains the same. Bisulfite Modification Kit - Whole Cell allows the user to perform DNA methylation analysis and modify DNA directly from cells or tissues within only 3 hours. Bisulfite Modification Kit - Whole Cell is specifically designed for DNA methylation research using minute amounts of starting materials including cells cultured in 96-well/384-well plates, tissue section samples, microdissection samples, tissue biopsy and early embryonic cells/oocytes. Modified DNA is eluted and suitable for real time MS-PCR as well as for all techniques currently used for the analysis of DNA methylation; including conventional MS-PCR, bisulfite sequencing, pyrosequencing, and methylation microarray.

Histone H4 (di-methyl R3) Quantification Kit (Colorimetric) is suitable for specifically measuring global histone H4 arginine 3 di-methylation from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including cultured cells and fresh tissues.

The DNA Methylation Kit features a simplified procedure that streamlines bisulfite treatment of DNA. The DNA Methylation Kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite during which cytosine is converted into uracil. Innovative desulphonation technologies eliminate otherwise cumbersome precipitations. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including library preparation for Next-generation sequencing, PCR amplification, endonuclease digestion, sequencing, microarrays, etc.

The kit is designed for the extraction of exceptionally pure total DNA from a variety of sources, including fresh or frozen animal tissues, cells, blood, bacteria, and other samples. It allows for the purification of DNA fragments with a maximum molecular weight of 50 kb, all without the need for hazardous solvents such as phenol or chloroform, as well as ethanol precipitation. The optimized buffer system efficiently and specifically binds DNA from the lysate to a silica-based centrifugal adsorption column. Through a two-step washing process, PCR inhibitors and other substances that might interfere with enzymatic reactions can be effectively removed. Ultimately, high-purity DNA can be eluted using a low-salt buffer or water, making it ready for direct use in various downstream applications, including enzyme digestion, PCR, Real-Time PCR, library construction, Southern Blot, molecular markers, and other experiments.

This product enables the rapid separation and purification of genomic DNA from 200-400 μL of fresh or frozen human or animal whole blood in just 15-20 minutes. The product does not require the use of proteinase K. After the whole blood is dissolved by Solution BFL1, the hemoglobin is removed by Solution BFL2 precipitation. The genomic DNA in the supernatant can be bound to the purification column. Subsequent washing with Buffer RP and Wash Buffer removes residual proteins on the membrane and PCR inhibitors. The genomic DNA is eluted by Elution Buffer and can be used in a wide range of molecular biology experiments immediately.

This kit is used for quantitative detection of telomerase (TE) activity in human serum, plasma, tissue homogenates, cell culture supernatants, urine, saliva, and other biological fluids.

The real-time fluorescent qPCR (SYBR Green Real-time PCR) method is extensively employed in scientific research due to its remarkable speed and precision in quantification. This kit offers a precise and straightforward means of analyzing mRNA expression. It leverages the meticulously designed optimal reaction system of SYBR Green qPCR Master Mix for fluorescent real-time quantitative PCR and Two-Step fluorescent real-time quantitative PCR, coupled with specific primers targeting the telomerase catalytic subunit gene TERT. This simplifies the experimental procedures and enhances overall efficiency. The Master Mix includes Maxima Hot Start Taq DNA Polymerase, dNTPs, an optimized PCR reaction buffer, and SYBR Green dye. The incorporation of Hot Start Taq DNA Polymerase results in PCR reactions with heightened yield, sensitivity, specificity, and the capability to detect as few as 10 copies of the target gene.

This assay utilizes the quantitative sandwich enzyme immunoassay technique. Initially, an antibody specific for TE is pre-coated onto a microplate. To perform the assay, standards, and samples are pipetted into the wells, where any TE present becomes bound by the immobilized antibody. After eliminating any unbound substances, a biotin-conjugated antibody that specifically recognizes TE is introduced into the wells. Following a subsequent washing step to remove unbound components, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. After another wash to eliminate any unbound avidin-enzyme reagent, a substrate solution is applied to the wells. In response, color development occurs, and the intensity of this color is directly proportional to the amount of TE initially bound in the first step of the assay. To complete the assay, the color development is halted, and the intensity of the color is measured, providing a quantifiable result reflecting the concentration of TE in the samples and standards.

DNA damage Assay Kit (AP sites, Colorimetric) provides a sensitive and specific method to monitor the formation of apurinic/apyrimidinic (AP) sites, one of the major types of DNA lesions. This DNA damage assay uses an APR (Aldehyde Reactive Probe) that reacts specifically with an aldehyde group on the open ring form of AP sites. AP sites are then tagged with biotin residues that can later be quantified using an streptavidin-enzyme conjugate that is easily detected by absorbance at OD450 nm. The kit has a detection sensitivity range of 4-40 AP sites per 1 x 10⁵ bp.

The DNA Damage Quantification Kit utilizes the ARP (Aldehyde Reactive Probe) reagent that reacts specifically with an aldehyde group which is the open ring form of the AP sites. After treating DNA containing AP sites with ARP reagents, AP sites are tagged with biotin residues, which can be quantified using avidin-biotin assay followed by a colorimetric detection. The kit provides the necessary reagents for convenient determination of abasic sites in purified DNA sample in 96-well plate format.

This kit can easily detect γH2AX as an indicator of DNA damage by the secondary antibody method. This kit contains all the reagents required for the experiment, which is easy to operate even for the first time users, and we will also introduce the literature and experimental examples of using γH2AX as an indicator for reference.DNA double-strand breaks are often seen in DNA damage, and H2AX (a histone subspecies of H2X), is rapidly and abundantly phosphorylated when DNA double-strand breaks occur. This phosphorylated H2AX (γH2AX), as a marker of DNA damage, is expected to be used as a means of evaluating the genotoxicity and carcinogenicity of chemicals, reactive oxygen species, ultraviolet light, and radiation. In recent years, the detection of γH2AX has also been used as an indicator for evaluating cellular senescence. This product uses anti-γH2AX antibodies produced in a monoclonal antibody laboratory.

This kit can easily detect γH2AX as an indicator of DNA damage by the secondary antibody method. This kit contains all the reagents required for the experiment, which is easy to operate even for the first time users, and we will also introduce the literature and experimental examples of using γH2AX as an indicator for reference.DNA double-strand breaks are often seen in DNA damage, and H2AX (a histone subspecies of H2X), is rapidly and abundantly phosphorylated when DNA double-strand breaks occur. This phosphorylated H2AX (γH2AX), as a marker of DNA damage, is expected to be used as a means of evaluating the genotoxicity and carcinogenicity of chemicals, reactive oxygen species, ultraviolet light, and radiation. In recent years, the detection of γH2AX has also been used as an indicator for evaluating cellular senescence. This product uses anti-γH2AX antibodies produced in a monoclonal antibody laboratory.