Assay Kits

Accurate and consistent quantification of telomere length is essential in many aspects of cell biology, such as chromosomal instability, DNA repair, senescence, apoptosis, cell dysfunctions, and oncogenesis. This product mainly uses relative quantitative qPCR to directly compare the average telomere length of the sample, that is, the copy number ratio (T/S) of the telomere repeat sequence (Tel) and the genome single copy gene (SCR) copy number (T/S) is used as the telomere relative length. The telomere primer set recognizes and amplifies telomere sequences. The single copy reference (SCR) primer set recognizes and amplifies the 100 bp-long regions on human chromosome 17 and serves as a reference for data normalization.

Accurate and consistent quantification of telomere length is essential in many aspects of cell biology, such as chromosomal instability, DNA repair, senescence, apoptosis, cell dysfunctions, and oncogenesis. The SMT003 kit is designed to measure the average telomere length directly in a population of human cells. The telomere primer set recognizes and amplifies telomere sequences. For data normalization, the single copy reference (SCR) primer set recognizes and amplifies 100 bp-long regions on human chromosome 17. To facilitate the calculation of absolute telomere length in target samples, the kit also contains a reference genomic DNA sample. This reference sample possesses a known telomere length, allowing for calibration and accurate determination of telomere length in the target samples.

This Assay Kit is specifically designed for the concurrent quantification of two important parameters, the average telomere length and the mitochondrial DNA (mtDNA) copy number, within a human cell population using quantitative polymerase chain reaction (qPCR) technology. Here's how it works: 1. Telomere Primer Set, This set of primers is designed to recognize and amplify telomere sequences within the cell population. 2. mtDNA Primer Set, This primer sets targets and amplifies one of the most highly conserved regions on human mtDNA. Importantly, it will not amplify any unintended sequences from nuclear genomic DNA. 3. Single Copy Reference (SCR) Primer Set, The SCR primer set is designed to recognize and amplify a specific 100 bp-long region located on human chromosome 17. It serves as a reference point for data normalization, enabling accurate comparisons between samples. To determine the telomere length and mtDNA copy number of target samples, a reference genomic DNA sample with known telomere length and mtDNA copy number is used as a benchmark. By comparing the qPCR results of the target samples to those of the reference, you can calculate the telomere length and mtDNA copy number for the samples of interest. This assay provides valuable information about cellular aging (telomere length) and mitochondrial function (mtDNA copy number) within the tested cell population, aiding in a wide range of research applications.

Accurate and consistent quantification of telomere length is essential in many aspects of cell biology, such as chromosomal instability, DNA repair, senescence, apoptosis, cell dysfunctions, and oncogenesis. This product utilizes relative quantitative PCR to determine the average telomere length of the given sample. The kit uses a single copy gene located on human chromosome 11 as the reference for data normalization. The relative telomere length is determined by calculating the copy number ratio (T/S) of telomere repeat sequences (Tel) to the single-copy reference (SCR).

This product constitutes a ready-to-use microwell and strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the assessment of the TEL2, telomere maintenance 2, homolog (TELO2) ELISA Kit target analytes within biological samples. The concentration gradients of the Kit standards or positive controls render a theoretical Kit detection range in biological research samples containing TELO2. The ELISA analytical biochemical technique of the Kit is based on TELO2 antibody-TELO2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TELO2 antigen targets in samples. It's important to note that this ELISA Kit is specifically tailored for detecting native, not recombinant TELO2.