Assay Kits

Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric) contains all reagents necessary for the quantification of global DNA methylation. In this assay, DNA is bound to strip-wells that are specifically treated to have a high DNA affinity. The methylated fraction of DNA is detected using capture and detection antibodies and then quantified colorimetrically by reading the absorbance in a microplate spectrophotometer. The percentage of methylated DNA is proportional to the OD intensity measured.

The m6A RNA Methylation Quantification Kit (Colorimetric) is a complete set of optimized buffers and reagents to colorimetrically quantify methylated N6-methyladenosine (m6A) in RNA. It is suitable for a direct detection of m6A RNA methylation status using total RNA isolated from any species such as mammals, plants, fungi, bacteria, and viruses. Be aware that m6A is present in mRNA and ribosomal RNA, and this kit detects m6A in total RNA, i.e. both mRNA and ribosomal RNA and any other RNA containing m6A. The amount of RNA for each assay can be 100 ng - 300 ng. For optimal quantification, the input RNA amount should be 200 ng, as the abundance of m6A is generally less tha 0.1% of total RNA. Chinese protocol available. See Protocols section below.

Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. Identifying the genetic targets of DNA binding proteins and knowing the mechanishm of protein-DNA interaction is important for understanding cellular processes. DNA-Protein Binding Assay Kit (Colorimetric) allows the user to investigate direct in vitro protein-DNA interaction, using dsDNA of choice, within 3 hours. DNA-Protein Binding Assay Kit (Colorimetric) is specifically suitable for detection of transcription factor activation in mammalian tissue and cell extracts.

DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quite recently, a novel modified nucleotide, 5-hydroxymethylcytosine (5-hmC), has been detected to be abundant in mouse brain and embryonic stem cells. It is a hydroxylated and methylated form of cytosine. Although it is still uncertain its specific role in epigenetics, it is believed it plays a role in DNA demethylation, chromatin remodeling and gene expression regulation. Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit enables the user to enrich hydroxymethated DNA using an antibody specific to hydroxymethylcytosine (5-hmC) to immunoprecipitate hydroxymethylated genomic DNA hMeDIP. The enriched hydroxymethylated fractions can be used in various downstream applications including PCR (hMeDIP-PCR) and microarrays (hMeDIP-chip) for investigating gene-specific hydroxymethylation in cells or tissues.

DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quantification of 5-mC content or global methylation status in cells such as tumor cells could provide very useful information for detection and analysis of cancer. Methylated DNA Quantification Kit (Colorimetric) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format. It can be paired to use with Hydroxymethylated DNA Quantification Kit (Colorimetric), which specifically quantifies 5-hmC, for simultaneous quantification of both methylated and hydroxymethylated DNA.

DNA methylation plays an essential role in biological processess through repression of transcription, while DNA demethylation is necessary, on the other hand, for epigenetic reprogramming of these genes. Active demethylation of DNA requires specific demethylase participation such as from MBD2. DNA demethylase (total) Activity Quantification Assay Kit allows the user to measure total DNA demethylase activity/inhibition at tremendously fast speeds and consistency; superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed for measuring total DNA demethylase activity/inhibition. This product is suitable for measuring DNA demethylase activity/inhibition from cultured cells/tissues or using purified enzyme/protein extracts.

DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It has been demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases, and that alterations in DNA methylation patterns are associated with cancer. Methylated DNA Immunoprecipitation (MeDIP) ChIP Kit enables the user to enrich methylated DNA directly from cells by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP). The enriched methylated fractions can then be used in various downstream applications including qualitative and quantitative PCR, as well as southern blot and DNA microarray.

Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. Identifying the genetic targets of DNA binding proteins and knowing the mechanishm of protein-DNA interaction is important for understanding cellular processes. DNA-Protein Binding Assay Kit (Fluorometric) allows the user to investigate direct in vitro protein-DNA interaction, using dsDNA of choice, within 3 hours. DNA-Protein Binding Assay Kit (Fluorometric) is specifically suitable for detection of transcription factor activation in mammalian tissue and cell extracts.

Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA enables the user to enrich methylated DNA by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP). The enriched methylated fractions can then be used in various downstream applications including qualitative and quantitative PCR, as well as southern blot and DNA microarray. DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It has been demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases, and that alterations in DNA methylation patterns are associated with cancer.

DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It has been demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases, and that alterations in DNA methylation patterns are associated with cancer. Methylated DNA Immunoprecipitation (MeDIP) ChIP Kit enables the user to enrich methylated DNA directly from various mammalian tissues by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP). The enriched methylated fractions can then be used in various downstream applications including qualitative and quantitative PCR, as well as southern blot and DNA microarray.

The Global DNA Hydroxymethylation Assay Kit is suitable for detecting global DNA hydroxymethylation levels using DNA isolated from any species including mammals, plants, fungi, bacteria, and viruses in a variety of forms including, but not limited to, cultured cells, fresh and frozen tissues, paraffin-embedded tissues, plasma/serum samples, and body fluid samples. In this assay, DNA is bound to strip-wells that are specifically treated to have a high DNA affinity. The hydroxymethylated fraction of DNA is detected using a 5-hmC mAb-based detection complex in a one-step manner and then quantified colorimetrically by reading the absorbance in a microplate spectrophotometer.

DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quite recently, a novel modified nucleotide, 5-hydroxymethylcytosine (5-hmC), has been detected to be abundant in mouse brain and embryonic stem cells. It is a hydroxylated and methylated form of cytosine. Although it is still uncertain its specific role in epigenetics, it is believed it plays a role in DNA demethylation, chromatin remodeling and gene expression regulation. The Hydroxymethylated DNA Quantification Kit (Colorimetric) is a complete set of optimized buffers and reagents to quantify global DNA hydroxymethylation by specifically measuring levels of 5-hydroxymethylcytosine (5-hmC) without cross-reactivity to methylcytosine and unmethylated cytosine. It can be paired to use with Methylated DNA Quantification Kit (Colorimetric), which specifically quantifies 5-mC, for simultaneously quantification of both methylated and hydroxymethylated DNA.

DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quite recently, a novel modified nucleotide, 5-hydroxymethylcytosine (5-hmC), has been detected to be abundant in mouse brain and embryonic stem cells. It is a hydroxylated and methylated form of cytosine. Although it is still uncertain its specific role in epigenetics, it is believed it plays a role in DNA demethylation, chromatin remodeling and gene expression regulation. Hydroxymethylated DNA Quantification Kit (Fluorometric) is a complete set of optimized buffers and reagents to quantify global DNA hydroxymethylation by specifically measuring levels of 5-hydroxymethylcytosine (5-hmC) without cross-reactivity to methylcytosine and unmethylated cytosine. It can be paired to use with EpiSeeker methylated DNA Quantification Kit (Fluorometric), which specifically quantifies 5-mC, for simultaneously quantification of both methylated and hydroxymethylated DNA.

DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quantification of 5-mC content or global methylation status in cells such as tumor cells could provide very useful information for detection and analysis of cancer. The Methylated DNA Quantification Kit (Fluorometric) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format. It can be paired to use with Hydroxymethylated DNA Quantification Kit (Fluorometric), which specifically quantifies 5-hmC, for simultaneously quantification of both methylated and hydroxymethylated DNA.

DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It is well demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases. A methylation-positive control is a must tool for a successful performance of gene methylation studies. Methylated Control DNA Kit provides a methylation-positive control for methylation studies. The kit includes the enzymatically methylated human genomic DNA and all components for DNA modification. Methylated human DNA needs to be modified before it is used as a positive control in MS-PCR. DNA modification reagents included in the kit are sufficient for modifying 5 µg of methylated human genomic DNA (10 modifications).

The MeDIP Ultra Kit is a complete set of optimized reagents to enrich and capture methylated DNA fragments in a convenient microplate-based format. The method, methylated DNA immunoprecipitation (MeDIP), uses a monoclonal antibody specific to 5-methylcytosine to immunoprecipitate methylated genomic DNA. The enriched methylated fractions can then be used for gene-specific DNA methylation analysis on a genome wide scale. The highly sensitive and specific format of the kit can use DNA isolated from various species. The methylated DNA that is enriched with this kit can be used for various downstream applications including qualitative and quantitative PCR (MeDIP-PCR), microarray (MeDIP-chip) and especially sequencing (MeDIP-seq). Starting Materials, Input Amount, & Expected Yield The starting material should be good quality purified DNA. The amount of DNA for each reaction can be 50 ng (approximately 10,000 cells) to 500 ng. For an optimal reaction, the input DNA amount should be 100-200 ng per well. The yielded methylated DNA is about 4 ng for 100 ng input DNA (4%), which is consistent with the expected percentage (4-5%) at which the highest sensitivity and specificity for enriched methylated DNA has been demonstrated by bisulfite sequencing.

DNMT Activity Quantification Kit (Colorimetric) allows the user to colorimetrically measure DNA methyltransferase activity or inhibition at extremely fast speed on a 96-strip well microplate. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT activity/inhibition experiment without the need for radioactivity or any special equipment. In the DNMT assay protocol, DNMT enzymes in a sample transfer methyl groups from Adomet to a DNMT DNA substrate coated onto microplate wells. The methylated DNA is bound with a 5-methylcytosine antibody. A secondary antibody binds the 5-methylcytosine antibody, and the enzyme-conjugated secondary antibody is detected using a chromogenic enzyme substrate.

The Histone H3 Modification Multiplex Assay Kit (Colorimetric) is a complete set of optimized reagents to detect and quantify up to 21 modified Histone H3 patterns simultaneously in a simple, ELISA-like format with use of a standard microplate reader. 

DNMT (DNA methylases) is a family of enzymes responsible for the addition of methyl groups on the cytosine ring at the 5' position of a CpG dinucleotide. The DNMT family is required for the establishment and maintenance of DNA methylation patterns. DNMT Activity Quantification Kit (Fluorometric) allows the user to fluorometrically measure DNA methyltransferase activity or inhibition at extremely fast speed on a 96-stripwell microplate. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT activity/inhibition experiment without the need for radioactivity or any special equipment.

Histone H3 (di-methyl K4) Quantification Kit (Colorimetric, Circulating) is a convenient package of tools designed to specifically measure circulating dimethyl histone H3K4 (H3K4me2) from biological fluid samples such as plasma and serum from human, mouse or rat. This kit only recognizes H3K4me2 with no cross-reactivity with unmodified H3 or other modifications at the same lysine site. The amount of plasma or serum for each assay can be 10-40 µL with an optimal amount of 30 µL.