Analysis of the Effects of Aging on Mitochondrial Permeability Transition Pore

Mitochondria play an important role in the cell aging process. Changes in calcium homeostasis and increased reactive oxygen species (ROS) production lead to the opening of mitochondrial permeability transition pores (MPTP), depolarization of the inner mitochondrial membrane, and decreased ATP production. The MPTP has been studied in detail for many years and is connected to aging and aging-related diseases, such as neurodegenerations, cardiovascular diseases, and cancer.

Fig. 1 Schematic representation of mPTP in brain aging and Alzheimer's disease. (Jia K and Du H, 2021)

The MPTP analysis in aging is of paramount importance in understanding age-related changes at the cellular level. At CD BioSciences, we offer comprehensive and highly advanced MPTP analysis services that provide invaluable insights into the role of mitochondrial function in the aging process.

Sample Types and Requirements

Our Workflow of Mitochondrial Permeability Transition Pore Analysis

• Sample collection and intact mitochondria isolation
  We begin by ensuring the proper collection and preservation of samples. After sample collection, we employ stringent protocols to isolate intact mitochondria from the samples.
• MPTP opening assays
  We utilize various techniques to assess MPTP opening in aging samples, such as measuring the mitochondrial swelling, calcium retention capacity, or release of mitochondrial proteins like cytochrome c. Our assays can provide insights into the sensitivity of the MPTP to inducers and how it responds to different experimental conditions.
• Electrophysiological studies
  We employ patch-clamp electrophysiology to directly measure the activity of the MPTP in isolated mitochondria or mitoplasts. Our approach provides detailed information about the conductance and regulation of the MPTP at the single-channel level.
• Data analysis and reporting
  Following the completion of the analyses, our team of experienced researchers meticulously analyze the data and generate comprehensive reports that highlight key findings and insights. This step ensures that our clients receive detailed and actionable information derived from the MPTP analysis in aging studies.

Our Advantages

• Advanced technological capabilities. We have access to cutting-edge technologies and state-of-the-art equipment for the evaluation of MPTP dynamics in aging samples. This includes advanced imaging techniques, fluorometric assays, and other analytical tools that can provide detailed insights into mitochondrial functions.
• Experience in sample handling and analysis. We are proficient in handling various sample types commonly encountered in aging research. Additionally, we can perform precise isolation of intact mitochondria and accurate analysis of MPTP activity.
• Quality assurance and reproducibility. We establish robust quality assurance processes to ensure the reproducibility and reliability of MPTP analysis results. Our services adhere to standardized protocols, rigorous validation procedures, and adherence to practices in the field.

At CD BioSciences, our MPTP analysis services represent cornerstones in unraveling the intricate interplay between mitochondrial function and aging. By harnessing the power of innovative methodologies and expert insights, we empower researchers to gain a comprehensive understanding of MPTP dynamics in aging, with the ultimate goal of developing targeted interventions to mitigate age-related mitochondrial impairments. If you are interested in our services, please feel free to contact us or make an online inquiry.

Reference

1. Jia K, Du H. Mitochondrial Permeability Transition: A Pore Intertwines Brain Aging and Alzheimer's Disease. Cells, 2021, 10 (3), 649.