Analysis of Telomeric Shelterin Complexes

Numerous factors impact the development and control of aging. One widely recognized molecular mechanism that contributes to aging is the gradual shortening of telomeres. The shelterin complex, which comprises six telomere-specific proteins, plays a crucial role in safeguarding chromosome ends. This complex is essential for protecting telomeres from degradation, preventing the activation of unwanted repair systems, regulating the activity of telomerase, and contributing to cellular senescence and age-related pathologies.

Fig .1 Telomere structure and the shelterin complex. (Mir SM, et al., 2020)

CD BioSciences is a reputable company specializing in biological research. With years of experience, our company is committed to offering comprehensive services for analyzing the telomeric shelterin complex during aging.

Overview of Our Telomere Shelterin Complex Analysis Services

Shelterin protein expression analysis

We offer the analysis of shelterin protein expression in aging. The shelterin complex, comprising proteins such as TRF1, TRF2, TIN2, POT1, TPP1, and RAP1, plays a crucial role in telomere maintenance and protection. Our advanced techniques enable precise quantification of shelterin protein expression levels, offering valuable insights into their regulation and potential alterations during the aging process.

Telomere protection assays

We help our clients assess the ability of the shelterin complex to protect telomeres from degradation and maintain genomic stability to understand aging-related telomere dysfunction. Our telomere protection assays provide critical data for elucidating its role in preserving telomere integrity during the aging process.

Shelterin complex interactome studies

We specialize in conducting comprehensive interactome studies to identify proteins that interact with the shelterin complex. By examining these interactions in the context of aging, we aim to elucidate the dynamic network of molecular associations involving the shelterin complex, shedding light on potential changes associated with aging-related telomere dysfunction.

Cellular senescence analysis

We offer tailored services for analyzing the influence of the shelterin complex on cellular senescence, providing valuable insights into the molecular mechanisms governing the aging process at the cellular level.

Workflow of Telomere Shelterin Complex Analysis in Aging

• Sample collection
  We obtain tissue samples from individuals of different ages, preferably from a specific tissue of interest such as blood, skin, or cells that are known to be affected by aging.
• Protein extraction
  We extract proteins from the collected tissue samples, specifically focusing on the components of the shelterin complex. This can be done using methods such as cell lysis followed by protein extraction and purification.
• Telomeric shelterin complex proteins analysis
  We perform Western blot analysis to detect and quantify the levels of shelterin complex proteins in the tissue samples, giving insights into changes in the abundance of shelterin complex components with aging.
  We utilize immunofluorescence staining techniques to visualize the localization and distribution of shelterin complex proteins within the cells of the tissue samples. Our services help clients understand any alterations in the spatial organization of the shelterin complexes with aging.
• Data analysis
  We analyze the results obtained from the above experiments to identify correlations between changes in shelterin complex components with aging. Statistical analyses and data visualization techniques can be used to interpret the findings.

Our Technical Tools for Telomere Shelterin Complex Analysis

• High-resolution mass spectrometers
• Western blot analysis
• Immunofluorescence staining

CD BioSciences, with its extensive expertise and cutting-edge analytical services, is committed to advancing the frontiers of aging by offering comprehensive solutions for the analysis of telomere shelterin complexes in aging. Through our dedicated efforts, we aim to contribute to the elucidation of the molecular mechanisms underpinning aging to facilitate the development of innovative strategies for promoting healthy aging. If you are interested in our services, please feel free to contact us or make an online inquiry.

Reference

1. Doksani Y. The Response to DNA Damage at Telomeric Repeats and Its Consequences for Telomere Function. Genes (Basel), 2019, 10 (4): 318.