Analysis of Telomerase Reverse Transcriptase Proteins

Telomerase is an RNA-protein complex; its main components are an RNA matrix for telomere synthesis, which also has a structural function, and telomerase reverse transcriptase (TERT). It plays a pivotal role in maintaining telomere length and cellular immortality. As the understanding of telomerase continues to expand, the development and application of telomerase activity assays have become increasingly important in aging research.

Fig.1 The core telomerase enzyme is minimally composed of the TERT protein and TR component. (Chen JJL and Podlevsky JD, 2016)

CD BioSciences, with decades of experience in biological research, offers a range of specialized analysis services to comprehensively understand TERT proteins in aging.

Overview of Our Telomerase Reverse Transcriptase Protein Analysis Services

Enzymatic activity assays

Our enzymatic activity assays, utilizing the quantitative sandwich enzyme immunoassay techniques, offer insight into the functional aspects of TERT proteins by assessing their enzymatic activity in aging cells and tissues. Our assays help in understanding the changes and alterations in TERT function with age, providing crucial information for aging research.

Protein expression analysis

By analyzing the expression of TERT proteins through techniques such as Western blot or immunohistochemistry, we can examine the expression levels of TERT in senescent cells or tissues, thus providing valuable information for understanding the role of TERT in the aging process.

Protein-protein interaction studies

Our protein-protein interaction studies have utilized techniques such as co-immunoprecipitation or yeast two-hybrid assays to reveal the complex network of TERT interactions during aging, leading to a comprehensive understanding of the molecular mechanisms of aging.

Workflow of Telomerase Reverse Transcriptase Protein Analysis

Our workflow for the analysis of TERT proteins in aging encompasses a systematic and meticulous approach.

• Sample collection and preparation
  We guide our clients in collecting aging cell cultures, tissue samples from age-related disease models, or patient-derived specimens. Proper handling and preparation of these samples are crucial to preserve the integrity of TERT proteins for subsequent analysis.
• Telomerase reverse transcriptase protein analysis
  Based on the specific research goals and requirements, we help our clients select and perform appropriate analysis techniques from a range of options such as enzymatic activity assays, protein expression analysis, and protein-protein interaction studies. Our processes are designed to provide insight into the functional aspects, expression levels, and molecular interactions of TERT proteins in the context of aging.
• Data interpretation and analysis
  Following the experimental analysis, we meticulously interpret and analyze the resulting data using the use of bioinformatics tools and advanced data analysis methods. Our goal is to draw meaningful conclusions and identify patterns or correlations within the data to contribute to the understanding of TERT proteins in aging.

Our Technical Tools for Telomerase Reverse Transcriptase Protein Analysis

• Enzyme-linked immunosorbent assay (ELISA)
• Western blot
• Immunohistochemistry
• Co-immunoprecipitation
• Yeast two-hybrid assays

CD BioSciences offers distinct advantages in the analysis of TERT proteins in aging research. With our extensive experience and expertise, we provide comprehensive support from sample collection to data interpretation. If you are interested in our services, please feel free to contact us or make an online inquiry.

Reference

1. Chen JJL and Podlevsky JD. Telomeres and Telomerase. Encyclopedia of Cell Biology, 2016, 418-25.