Analysis of Intercellular Communication in Senescence

Intercellular communication plays a fundamental role in coordinating cellular activities and maintaining tissue homeostasis. A range of molecular signaling mechanisms facilitates intercellular communication during senescence to enable information exchange between cells. Senescent cells are highly proactive and communicate with neighboring cells via various means of intercellular communication including but not limited to the senescence-associated secretory phenotype (SASP).

Fig. 1 Intercellular communication in senescence and aging. (Fafián-Labora JA and O'Loghlen A, 2020)

Over time, modifications in these communication networks can lead to variations in the function of individual cells and tissues. CD BioSciences specializes in appraising the intercellular communication mechanisms relevant to senescence, a critical event involved in the aging of cells and the development of age-related diseases.

Types of Analyzable Intercellular Communication During Senescence

Direct cell-cell contact

We employ advanced microscopy techniques, such as confocal microscopy and live cell imaging, to observe and analyze intercellular interactions in senescent cells. Our analysis focuses on structures that facilitate direct communication, namely gap junctions and tunneling nanotubes. Moreover, we also observe altered intercellular communication and senescence-associated phenotypes resulting from the disruption of direct cell-cell contact.

Paracrine signaling

We utilize proteomic and transcriptomic approaches, such as mass spectrometry and RNA sequencing, to identify and quantify the secreted factors involved in intercellular communication during senescence. Our services include but are not limited to, the release of signaling molecules such as growth factors, cytokines, and chemokines by cells. Our analysis allows clients to understand the composition of the SASP and other secretomes associated with senescent cells.

Exosomes and extracellular vesicles approach

We leverage specialized techniques, such as nanoparticle tracking analysis and electron microscopy, to isolate and characterize exosomes and extracellular vesicles discharged by senescent cells. Based on this, we offer insight into the cargo and functional properties of these vesicles, exposing their function in intercellular communication. We investigate the senescence process, whereby exosomes propagate senescence-related signals and regulate cellular senescence in an autocrine and paracrine manner.

Our Integrated Intercellular Communication Analysis Services

Intercellular signaling pathway profiling

We offer services to profile the activation and modulation of intercellular signaling pathways such as Wnt, Notch, TGF-β, and others in aging cells or tissues using techniques like phosphoproteomics and pathway-specific reporter assays.

Computational modeling of intercellular communication

We offer computational modeling services to simulate and predict the dynamics of intercellular signaling networks in aging tissues, enabling the identification of key signaling nodes and potential intervention points.

Workflow of Analyzing Intercellular Communication in Aging Studies

• Experimental design
  We design experiments to investigate intercellular communication in aging. We help our clients select model systems (e.g., cultured cells, animal models), experimental manipulations (e.g., aging-related stressors), and appropriate controls.
• Experimental execution
  We perform cellular and molecular techniques to analyze intercellular communication. Our services may include cell culture studies, co-culture systems, and the use of molecular tools such as fluorescent probes, immunostaining, and gene expression analysis. We also utilize omics approaches to assess changes in intercellular communication during aging.
• Data analysis and interpretation
  We integrate data from various experimental techniques to gain a holistic understanding of intercellular communication in aging. If requested, we can build a comprehensive model of intercellular signaling in the aging process.

CD BioSciences, with its extensive experience, advanced technologies, and customer-centric approach, offers comprehensive analysis services that provide valuable insights into intercellular communication mechanisms in senescence. If you are interested in our services, please feel free to contact us or make an online inquiry.

Reference

1. Fafián-Labora JA, O'Loghlen A. Classical and Nonclassical Intercellular Communication in Senescence and Ageing. Trends Cell Biol. 2020, 30 (8): 628-639.