Analysis of Dysregulated Nutrient Sensing During Aging

Nutrient perception refers to the ability of cells to recognize and respond to energy substrates, such as glucose, fatty acids, and ketones. Nutrient sensing pathways play a crucial role in regulating cellular metabolism, growth, and survival. Dysregulated nutrient sensing is a hallmark of aging and is associated with various age-related diseases, including metabolic disorders, neurodegenerative diseases, and cancer. At CD BioSciences, we are committed to providing comprehensive analysis services for studying dysregulated nutrient sensing during aging.

Fig. 1 A map of the signaling pathways involved in dysregulated nutrient-sensing and their relationship to aging.

Critical Nutrient Sensing Pathway Analysis Services We Offer

Analysis of insulin/IGF-1 signaling pathway in aging

In mammals, insulin/IGF-1 signaling is an important coordinator of nutrient availability with energy homeostasis and metabolic processes, which is activated by insulin-like peptide (ILP) ligands in response to nutrient availability.

Using advanced molecular techniques, we examine crucial constituents of the insulin/IGF-1 signaling pathway, encompassing insulin receptors, insulin-like growth factor receptors, downstream signaling molecules, and transcription factors. By investigating the expression levels, post-translational modifications, and activity of these constituents, we can detect anomalous signaling events and potential treatment targets for age-related metabolic disorders.

Analysis of the mTOR pathway in aging

The mTOR pathway serves as a pivotal controller of cellular growth and metabolism by regulating nutrient availability and energy status. We employ cutting-edge methods to examine the activity, protein expression, and positioning of crucial elements like mTOR complexes, upstream regulators, and downstream effectors within the mTOR pathway. Our services provide a means to gain insight into the underlying mechanisms of age-related diseases and to identify potential therapeutic targets for intervention.

Analysis of the AMPK pathway in aging

The AMP-activated protein kinase (AMPK) pathway is a cellular energy sensor that regulates energy homeostasis and metabolism. Through advanced molecular techniques, we analyze the activity, protein expression, and phosphorylation status of key components of the AMPK pathway, including AMPK subunits, upstream kinases, and downstream targets. Based on studying the dysregulation of the AMPK pathway, we can gain insights into the metabolic changes associated with aging and identify potential therapeutic targets for age-related metabolic disorders.

Our Workflow of Analyzing Dysregulated Nutrient Sensing During Aging

• Study design
  We collaborate closely with our clients to design studies that address their specific research questions and objectives. We help our clients select aging cellular models and animal models to study the impact of dysregulated nutrient sensing on aging-related phenotypes.
• Molecular analysis
  Firstly, we help our clients analyze and identify the specific nutrient-sensing pathways (e.g., mTOR, insulin/IGF-1, AMPK, sirtuins) based on their relevance to aging and associated dysregulation. Then, we measure the expression and activity of key proteins and metabolites involved in the selected nutrient-sensing pathways. We also investigate genetic and epigenetic alterations and metabolic profiling associated with dysregulated nutrient sensing during aging.
• Data analysis and interpretation
  We utilize bioinformatic tools and systems biology approaches to analyze complex datasets. We provide in-depth data interpretation, including pathway analysis, functional annotation, and identification of key regulatory networks associated with dysregulated nutrient sensing during aging.

Deliverables for Analyzing Dysregulated Nutrient Sensing in Aging Studies

• Methodology. We provide a detailed description of the analytical methods and techniques used to assess nutrient-sensing deregulation. We outline the experimental approaches, sample preparation, data collection methods, and any specific considerations relevant to the analysis.
• Data analysis. We present the experimental data, including quantitative results such as gene expression profiles, expression and activity of key proteins and metabolites, or other relevant measurements. The data analysis section may include tables, graphs, and statistical analyses to illustrate the findings effectively.
• Interpretation and discussion. Our reports include a thorough interpretation and discussion of the results, highlighting key findings and their implications in aging and age-related diseases.
• Actionable recommendations. Based on the analysis results, we offer actionable recommendations for further research or therapeutic development, including potential therapeutic targets and strategies for modulating dysregulated nutrient sensing pathways.

CD BioSciences provides comprehensive analysis services with a focus on unraveling the complexities arising from dysregulated nutrient sensing during aging. If you are interested in our services, please feel free to contact us or make an online inquiry.

References

1. López-Otín C, et al. The hallmarks of aging. Cell. 2013, 153 (6): 1194-217.
2. Fernandes SA, Demetriades C. The Multifaceted Role of Nutrient Sensing and mTORC1 Signaling in Physiology and Aging. Front Aging. 2021, 2: 707372.