Analysis of Age-Related Chromatin Remodeling

Chromatin remodeling is defined as a series of genome-wide changes in the nuclear architecture that can be recognized at the level of specific chromosomes or chromosome domains, such as centromeres. Significant chromatin structural remodeling has been identified during cellular senescence, from histone component and modification changes to alterations of the chromatin compartments and topologically associating domains (TADs). CD BioSciences presents cutting-edge services in analyzing age-related chromatin remodeling, unraveling the complexities of aging, and developing targeted interventions to promote healthy aging.

Fig. 1 Summary of the changes in the chromatin organization and function in aged cells. (Purohit JS and Chaturvedi MM, 2016)

Overview of Our Chromatin Remodeling Analysis Services

At CD BioSciences, we offer a comprehensive range of services to analyze age-related chromatin remodeling. We analyze the process of chromatin remodeling in eukaryotes in terms of two major factors, histone-modifying enzymes and ATP-dependent chromatin remodeling complexes.

Analysis of ATP-dependent chromatin-remodeling complexes in aging

• SWI/SNF (switch defective/sucrose non-fermenting) family
  CD BioSciences can analyze the activity and composition of SWI/SNF complexes in aging cells and tissues. The activity of SWI/SNF complexes declines with age, which can lead to changes in gene expression patterns and contribute to the aging phenotype.
  Additionally, we discover mutations or dysfunction in SWI/SNF components that have been implicated in various age-related diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases.
• ISWI (imitation switch) group of chromatin-remodeling complexes
  We evaluate the functional impact of ISWI complexes on lifespan, gene expression patterns, and various age-associated cellular processes. Our services are available in various model organisms, such as worms, flies, and mice. In addition, we provide insights into the impact of the ISWI complex on maintaining genome stability, which is critical for preventing the accumulation of age-related DNA damage.
• CHD (chromodomain, helicase, DNA binding) chromatin-remodeling factors
  We provide analysis services for dysregulation of CHD chromatin-remodeling factors in various aspects of aging, including cellular senescence, DNA damage response, and transcriptional regulation. It has been proven associated with various age-related disorders, including neurodevelopmental and neurodegenerative diseases.
• INO80 (inositol requiring) factors
  We offer analyses of the functions and mechanisms of INO80 factors in age-related chromatin remodeling, which are involved in neurodegenerative diseases and aging-related phenotypes. We analyze its different types of remodeling activities, such as nucleosome relocation, expulsion, substitution, and histone exchange with histone variants, mediated by replication-independent pathways.

Analysis of histone-modifying enzymes in aging

CD BioSciences employs state-of-the-art techniques to examine the activity and expression levels of histone-modifying enzymes in aging cells and tissues, such as histone deacetylase and methyltransferase. By doing so, we can gain comprehensive insights into the alterations in histone modifications that occur during aging and their implications for age-related chromatin remodeling.

Our Assays for Chromatin Remodeling

We have a variety of methods for detecting chromatin remodeling, including chromatin immunoprecipitation (ChIP), chromosome conformation capture (3C), and fluorescence in situ hybridization (FISH). As high-throughput sequencing and single-cell technologies evolve, we are constantly improving our assays. By utilizing these tools, we can perform comprehensive investigations into age-related chromatin remodeling, achieving a deeper understanding of the underlying mechanisms.

CD BioSciences excels in offering comprehensive analysis services in the field of age-related chromatin remodeling, encompassing ATP-dependent chromatin-remodeling complexes and histone-modifying enzymes. If you are interested in our services, please feel free to contact us or make an online inquiry.

Reference

1. Purohit JS, Chaturvedi MM. Chromatin and Aging. Topics in Biomedical Gerontology, 2016, 18: 205-41.