CAT. NO.: SMT007
| General Info | |
|---|---|
| Size | 100 tests |
| Product Overview | This Assay Kit is specifically designed for the concurrent quantification of two important parameters, the average telomere length and the mitochondrial DNA (mtDNA) copy number, within a human cell population using quantitative polymerase chain reaction (qPCR) technology. Here's how it works: 1. Telomere Primer Set, This set of primers is designed to recognize and amplify telomere sequences within the cell population. 2. mtDNA Primer Set, This primer sets targets and amplifies one of the most highly conserved regions on human mtDNA. Importantly, it will not amplify any unintended sequences from nuclear genomic DNA. 3. Single Copy Reference (SCR) Primer Set, The SCR primer set is designed to recognize and amplify a specific 100 bp-long region located on human chromosome 17. It serves as a reference point for data normalization, enabling accurate comparisons between samples. To determine the telomere length and mtDNA copy number of target samples, a reference genomic DNA sample with known telomere length and mtDNA copy number is used as a benchmark. By comparing the qPCR results of the target samples to those of the reference, you can calculate the telomere length and mtDNA copy number for the samples of interest. This assay provides valuable information about cellular aging (telomere length) and mitochondrial function (mtDNA copy number) within the tested cell population, aiding in a wide range of research applications. |
| Storage Conditions | Store the GoldNStart TaqGreen qPCR master mix in the dark at -20°C in a manual defrost freezer, the primers and the reference genomic DNA sample at -20°C in a manual defrost freezer, and the nuclease-free H2O at 4°C. |
| Reactivity | Human |
| Shipping Conditions | Dry Ice |
| Product Details | |
|---|---|
| Assay Type | qPCR |
| Samples | Genomic DNA |
| Components | 2×GoldNStart TaqGreen qPCR master mix Telomere primer set, lyophilized Human mtDNA primer set, lyophilized Human single copy reference (SCR) primer set, lyophilized Nuclease-free H2O Reference Human genomic DNA sample |
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