CAT. NO.: SAT-002
| General Info | |
|---|---|
| Size | 100 tests |
| Product Overview | Three-dimensional (3D) cell cultures are artificially-created environments, where cells are allowed to grow or interact with their surroundings in a 3D fashion. 3D cell cultures improve the function, differentiation and viability of cells and recapitulate the in vivo microenvironment as compared to conventional 2D cell cultures. 3D matrices provide a physiologically relevant screening platform by mimicking the in vivo responses for many cell types including cancer and stem cells. This is specifically important in developmental morphogenesis, pharmacology, drug metabolism and drug toxicity studies. Additionally, quantification of the number of viable cells is an indispensable tool in in vitro screening in these studies. However, with the use of 3D matrices, some protease-based dissociation methods don’t completely dissolve the matrices or the cell aggregates, which may alter the result of the in vitro viability assessment quantitatively. 3D Culture Cell Viability Assay Kit provides a standardized colorimetric method for sensitive quantification of viable cells that can detect as high as 250,000 viable cells and as low as 1000 viable cells in each well. The absorbance is measured at 460 nm. The measured intensity is proportional to the number of viable cells. Further, the kit comes with an optimized and gentle non-enzymatic dissociation solution for the recovery of viable and dead cells from spheroids in matrices and scaffolds. This assay kit provides an easy-to-use, non-radioactive, high-throughput method for characterizing and screening cell viability and cytotoxicity. |
| Notes | • Analysis of cytotoxic/cytostatic compounds that affect cell growth and spheroid formation, such as anticancer drugs, toxic agents and other pharmaceuticals. • Measurement of cell viability in response to growth factors, cytokines, mitogens and nutrients. • Matrix and spheroid dissociations from 3D cell culture for cell growth assessment. |
Our customers have direct access to our experts and give timely feedback to any online inquiries. If you are interested in our products, please contact us.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
CD BioScience is a research service provider focused on aging research, specializing in providing research on aging mechanisms, preclinical drug discovery and development, and the development of healthcare and skincare products.
Copyright © 2024 CD BioSciences. All rights reserved.