CAT. NO.: AF018
| Product Details | |
|---|---|
| Synonym(s) | Dichlorofluorescein, DCF |
| Empirical Formula (Hill Notation) | C20H10Cl2O5 |
| CAS Number | 76-54-0 |
| Molecular Weight | 401.2 |
| MDL Number | MFCD00005047 |
| PubChem Substance ID | 57649439 |
| Size | 5 g/25 g |
| Assay | ≥90% (T) |
| Fluorescence | λex 504 nm; λem 529 nm in 0.1 M Tris pH 8.0 |
| SMILES string | Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl |
| Description | |
|---|---|
| 2′,7′-Dichlorofluorescein, also known as DCF is a fluorescent dye. The DCF fluorescence excitation is at 498nm with emission at 522nm. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured. | |
| Application | |
|---|---|
| 2′,7′-Dichlorofluorescein is used as fluorescent probe to measure ROS (Reactive Oxygen Species) for biological studies. (3) It may also be used as a dip reagent for the fluorometric determination of vicinal diols, glycosides, and phenols. | |
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