14-3-3 η Antibody
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14-3-3 η Antibody

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CAT. NO.: AMA-0651

Target Information
UniProt ID Q04917
Product Details
Size 100 µL
Reactivity Human, Mouse, Rat, Monkey
Storage Store at -20°C. Do not aliquot the antibody.
Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human 14-3-3 η. Antibodies are purified by protein A and peptide affinity chromatography.
Usage
Application WB, IP
Product Usage Information Dilution Western Blotting (1:1000); Immunoprecipitation (1:50)
MW (Target) 28 kDa
Background
The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation independent interactions has been observed 14-3-3 binding masks specific sequences of the target protein, and therefore, modulates target protein localization, phosphorylation state, stability, and molecular interactions 14-3-3 proteins may also induce target protein conformational changes that modify target protein function Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes.

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